Determination of Thymol in Mouthwash by Capillary Electrophoresis

Zahkary G. Barone, Thompson Rivers University

Abstract

Thymol is a natural monoterpene phenol derivative of p-cymene found in oil of thyme and extracted from various plants which has strong antiseptic properties. Thymol is listed as a medical ingredient in mouthwash and some toothpastes as it contains active antiseptic ingredients. Many brands of mouthwash state that thymol fights tooth decay and infections, and it was found that mouthwash containing thymol was effective at preventing the initiation of dental caries – a pathological process of destruction of tooth structure by microorganisms. Thymols antibacterial activity is caused by inhibiting growth and lactate production and by decreasing cellular glucose uptake. This study was conducted to separate and quantify the amount of thymol (C10H14O) present in mouthwash by capillary electrophoresis (CE), and to compare the results obtained to the manufacturers claim. Nine samples of mouthwash from four companies (Listerine, Crest, Equate, and Life) were measured to determine their concentration of thymol. Quantifying the amount of thymol contained in mouthwash could aid in ensuring the appropriate amount is present to effectively prevent dental decay. CE was chosen as it is an extremely sensitive instrument that is proficient at separating analytes. Analytes are separated according to ionic mobility via an applied voltage; an ions mobility depends on its size, charge and viscosity. Data is collected as electropherograms which plot the absorbance of the analytes against their migration time which allows for the quantification of the amount of thymol present in the samples.

 
Mar 31st, 1:19 PM Mar 31st, 1:25 PM

Determination of Thymol in Mouthwash by Capillary Electrophoresis

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Thymol is a natural monoterpene phenol derivative of p-cymene found in oil of thyme and extracted from various plants which has strong antiseptic properties. Thymol is listed as a medical ingredient in mouthwash and some toothpastes as it contains active antiseptic ingredients. Many brands of mouthwash state that thymol fights tooth decay and infections, and it was found that mouthwash containing thymol was effective at preventing the initiation of dental caries – a pathological process of destruction of tooth structure by microorganisms. Thymols antibacterial activity is caused by inhibiting growth and lactate production and by decreasing cellular glucose uptake. This study was conducted to separate and quantify the amount of thymol (C10H14O) present in mouthwash by capillary electrophoresis (CE), and to compare the results obtained to the manufacturers claim. Nine samples of mouthwash from four companies (Listerine, Crest, Equate, and Life) were measured to determine their concentration of thymol. Quantifying the amount of thymol contained in mouthwash could aid in ensuring the appropriate amount is present to effectively prevent dental decay. CE was chosen as it is an extremely sensitive instrument that is proficient at separating analytes. Analytes are separated according to ionic mobility via an applied voltage; an ions mobility depends on its size, charge and viscosity. Data is collected as electropherograms which plot the absorbance of the analytes against their migration time which allows for the quantification of the amount of thymol present in the samples.