Presentation Title

Determination of Major Components in Propolis by Capillary Electrophoresis

Format of Presentation

Poster to be presented the Friday of the conference

Abstract

Propolis is a viscous substance collected by honeybees and used as a hive protector. Its bioactive properties such as antibacterial, antioxidant, and antifungal activities are what makes it a popular natural remedy that has long been used by humans in pharmacological settings. Essential components in propolis that are responsible for its beneficial biological properties are polyphenols which include flavonoids and phenolic acids. These compounds are proven to act as inhibitors of specific enzymes, hormone and neurotransmitter mimetics and scavenge for free radicals. A rapid and sensitive method was developed to determine the major components in propolis using capillary electrophoresis [CE]. Method parameters such as the wavelength monitored by the UV detector, pH and concentration of the buffer, the voltage and injection time on the CE were optimized. Under the optimum conditions, four flavonoids and one phenolic acid were separated within 35 min in a borate buffer with sodium dodecyl sulfate (pH 9.0). This method was successfully used for the analysis of propolis tinctures without an extraction step. Future work would include the identification and quantification of other flavonoids and phenolic acids in differently sourced propolis and to test their bioactivity against certain harmful bacteria and fungi.

Department

Biological Sciences

Faculty Advisor

Naowarat Cheeptham

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Determination of Major Components in Propolis by Capillary Electrophoresis

Propolis is a viscous substance collected by honeybees and used as a hive protector. Its bioactive properties such as antibacterial, antioxidant, and antifungal activities are what makes it a popular natural remedy that has long been used by humans in pharmacological settings. Essential components in propolis that are responsible for its beneficial biological properties are polyphenols which include flavonoids and phenolic acids. These compounds are proven to act as inhibitors of specific enzymes, hormone and neurotransmitter mimetics and scavenge for free radicals. A rapid and sensitive method was developed to determine the major components in propolis using capillary electrophoresis [CE]. Method parameters such as the wavelength monitored by the UV detector, pH and concentration of the buffer, the voltage and injection time on the CE were optimized. Under the optimum conditions, four flavonoids and one phenolic acid were separated within 35 min in a borate buffer with sodium dodecyl sulfate (pH 9.0). This method was successfully used for the analysis of propolis tinctures without an extraction step. Future work would include the identification and quantification of other flavonoids and phenolic acids in differently sourced propolis and to test their bioactivity against certain harmful bacteria and fungi.