Presentation Title

Investigating the Interaction Between Bisphenol-S and Human Serum Albumin Using Affinity Capillary Electrophoresis

Format of Presentation

Poster to be presented the Friday of the conference

Abstract

Bisphenol S (BPS) has been identified as a suitable subsitute to bisphenol A (BPA) due to its high stability and other features. Recent studies have shown that BPS might have a similar effect as BPA by acting as a potential endocrine-disrupting chemical with a harmful effect on human and animal health. Exposure to BPS has a negative effect on the reproductive, endocrine and cardiovascular system. Human serum albumin (HSA) is the most abundant protein in the human blood plasma and is involved in the transport of various fatty acids, hormones and other molecules around the body. Due to its function and abundance, it is the Ideal protein for the study of the health implications of the new substitute (BPS).

This study aims to determine the interaction between BPS and HSA using affinity capillary electrophoresis (ACE) and nuclear magnetic resonance (NMR) spectroscopy. From the changes in migration time at different concentrations of the additive (HSA), ACE will yield a binding constant that represents the strength of the interaction. A similar study is done using NMR spectroscopy. A change in the chemical shift upon the addition of increasing concentration of BPS is modelled to calculate the binding constant and give an indication of the strength of interaction. The result of this interaction study will provide more information on the health effects of BPS.

Department

Chemistry

Faculty Advisor

Kingsley Donkor

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Investigating the Interaction Between Bisphenol-S and Human Serum Albumin Using Affinity Capillary Electrophoresis

Bisphenol S (BPS) has been identified as a suitable subsitute to bisphenol A (BPA) due to its high stability and other features. Recent studies have shown that BPS might have a similar effect as BPA by acting as a potential endocrine-disrupting chemical with a harmful effect on human and animal health. Exposure to BPS has a negative effect on the reproductive, endocrine and cardiovascular system. Human serum albumin (HSA) is the most abundant protein in the human blood plasma and is involved in the transport of various fatty acids, hormones and other molecules around the body. Due to its function and abundance, it is the Ideal protein for the study of the health implications of the new substitute (BPS).

This study aims to determine the interaction between BPS and HSA using affinity capillary electrophoresis (ACE) and nuclear magnetic resonance (NMR) spectroscopy. From the changes in migration time at different concentrations of the additive (HSA), ACE will yield a binding constant that represents the strength of the interaction. A similar study is done using NMR spectroscopy. A change in the chemical shift upon the addition of increasing concentration of BPS is modelled to calculate the binding constant and give an indication of the strength of interaction. The result of this interaction study will provide more information on the health effects of BPS.