Presentation Title

Assessing the Relationship between Cluster of Differentiation 34 Positive (CD34+) Stem Cells and Platelet Microvesicles during High-Intensity Exercise

Format of Presentation

Poster to be presented the Friday of the conference

Abstract

Platelet microvesicles (PMVs) originate from platelets, have a submicron diameter, and transport biomolecules. PMV levels are elevated during high-intensity exercise (HIE) and previous work shows PMV-mediated angiogenesis through endothelial cell proliferation. Cluster of differentiation 34 positive (CD34+) stem cell levels are also elevated after HIE. Whether this HIE-related increase in CD34+ stem cells results from PMV stimulation is unknown. The present study investigates this relationship through platelet function inhibition by non-steroidal anti-inflammatory drug (NSAID) ingestion. Ten participants performed an initial test to determine aerobic fitness (VO2max), and two experimental trials (NSAID and control). For the VO2max test, participants performed a step-wise ramp protocol (4W every 10 seconds) until volitional exhaustion. Blood pressure, breath-by-breath gas exchange, and heart rate were recorded and the data analyzed using the Beaver and Wasserman method and COSMED OMNIA software. Power output set points for the two experimental trials were calculated using one-third the difference between the first and second ventilatory thresholds. For NSAID and control trials, participants ingested either 600 mg of ibuprofen or a placebo two hours prior to 45 minutes of cycling. Nine mL of blood was drawn at 0, 45, 90, 135 minutes. Blood was stained with CD34, CD45, and CD133 fluorescent antibodies for progenitor cell enumeration using flow cytometry. Other blood fractions were centrifuged and the plasma frozen at -80°C for future PMV enumeration. PMV and CD34+ progenitor cell counts from both experimental trials will be compared to assess if a relationship exists between PMVs and CD34+ progenitor cells during HIE.

Department

Biological Sciences

Faculty Advisor

Mark Rakobowchuk

This document is currently not available here.

Share

COinS
 

Assessing the Relationship between Cluster of Differentiation 34 Positive (CD34+) Stem Cells and Platelet Microvesicles during High-Intensity Exercise

Platelet microvesicles (PMVs) originate from platelets, have a submicron diameter, and transport biomolecules. PMV levels are elevated during high-intensity exercise (HIE) and previous work shows PMV-mediated angiogenesis through endothelial cell proliferation. Cluster of differentiation 34 positive (CD34+) stem cell levels are also elevated after HIE. Whether this HIE-related increase in CD34+ stem cells results from PMV stimulation is unknown. The present study investigates this relationship through platelet function inhibition by non-steroidal anti-inflammatory drug (NSAID) ingestion. Ten participants performed an initial test to determine aerobic fitness (VO2max), and two experimental trials (NSAID and control). For the VO2max test, participants performed a step-wise ramp protocol (4W every 10 seconds) until volitional exhaustion. Blood pressure, breath-by-breath gas exchange, and heart rate were recorded and the data analyzed using the Beaver and Wasserman method and COSMED OMNIA software. Power output set points for the two experimental trials were calculated using one-third the difference between the first and second ventilatory thresholds. For NSAID and control trials, participants ingested either 600 mg of ibuprofen or a placebo two hours prior to 45 minutes of cycling. Nine mL of blood was drawn at 0, 45, 90, 135 minutes. Blood was stained with CD34, CD45, and CD133 fluorescent antibodies for progenitor cell enumeration using flow cytometry. Other blood fractions were centrifuged and the plasma frozen at -80°C for future PMV enumeration. PMV and CD34+ progenitor cell counts from both experimental trials will be compared to assess if a relationship exists between PMVs and CD34+ progenitor cells during HIE.