Presentation Title

Investigating the Interaction between Bisphenol A and Human Serum Albumin using Affinity Capillary Electrophoresis

Format of Presentation

Poster to be presented the Friday of the conference

Presenter Information

Oluwafemi Francis OgunyemiFollow

Abstract

Bisphenol A (BPA) is a chemical compound used in the production of polycarbonate plastics which are used in containers that store food and beverages. Studies over the years show that BPA is an endocrine disruptor and is believed to have a harmful effect on human health. Human serum albumin (HSA) is the most abundant protein in human blood plasma and is involved in transport of various fatty acids, hormones and other molecules around the body. Due to its function and abundance, it is the ideal protein for the study of the health implications of BPA.

For this project, affinity capillary electrophoresis (ACE) will be used to investigate the type and strength of the interaction between BPA and HSA. A prepared concentration of BPA will be run in different buffers containing varying concentrations of HSA. The first run is done without the HSA to determine the migration time of BPA. A change in the migration time will be observed with the introduction of HSA because of the complex formed as a result of the interaction between both molecules. A binding constant which represents the strength of the interaction will be determined from the changes in migration time at different concentrations of the HSA additive. The type of interaction will be obtained from the thermodynamic parameters (ΔG, ΔS, ΔH) calculated from the data obtained. The result obtained from this study will provide more information on the effect of BPA on human health.

Department

Chemistry

Faculty Advisor

Kingsley Donkor

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Investigating the Interaction between Bisphenol A and Human Serum Albumin using Affinity Capillary Electrophoresis

Bisphenol A (BPA) is a chemical compound used in the production of polycarbonate plastics which are used in containers that store food and beverages. Studies over the years show that BPA is an endocrine disruptor and is believed to have a harmful effect on human health. Human serum albumin (HSA) is the most abundant protein in human blood plasma and is involved in transport of various fatty acids, hormones and other molecules around the body. Due to its function and abundance, it is the ideal protein for the study of the health implications of BPA.

For this project, affinity capillary electrophoresis (ACE) will be used to investigate the type and strength of the interaction between BPA and HSA. A prepared concentration of BPA will be run in different buffers containing varying concentrations of HSA. The first run is done without the HSA to determine the migration time of BPA. A change in the migration time will be observed with the introduction of HSA because of the complex formed as a result of the interaction between both molecules. A binding constant which represents the strength of the interaction will be determined from the changes in migration time at different concentrations of the HSA additive. The type of interaction will be obtained from the thermodynamic parameters (ΔG, ΔS, ΔH) calculated from the data obtained. The result obtained from this study will provide more information on the effect of BPA on human health.