Presentation Title

Anti-Pseudogymnoascus destructans (Pd) Activities from Canadian Western Bat Wings

Format of Presentation

Poster to be presented the Friday of the conference

Abstract

White-nose syndrome, an infectious fungal illness in bats caused by the psychrophilic Pseudogymnoascus destructans (Pd), has decimated North American hibernating bat populations at an unprecedented rate. The fungus establishes on the surface of bats during their hibernation cycle, resulting in their premature arousal from torpour and depletion of vital fat stores required for hibernation. As some species of bacteria have an antagonistic relationship with fungi, the elimination of Pd through the native microbiota of bats may serve as a non-invasive prevention method that limits the disruption of hibernacula ecosystems. In this study, bacteria found on batwings were isolated and screened for anti-Pd activity. These bacteria could be used to develop a probiotic powder to be placed in hibernacula and on bat colonies in hope to help prevent bats from WNS. Batwing swabs were obtained from captured bats between the summer of 2016 and the summer of 2018. The swabs were plated on blood agar, nutrient agar (NA), Reasoner’s 2A (R2A) agar, potato dextrose agar (PDA), and Wallerstein Laboratory (WL) agar. Culturable bacteria were re-streaked and incubated at 25-30°C for 24-48 hours to obtain pure cultures. Using the agar diffusion method, we screened the anti-Pd activity for our isolates on Sabouraud Dextrose agar (SDA) at 14°C for 28 days. Among the 585 bacterial isolates tested, five showed full inhibition and 34 showed partial inhibition. The anti-Pd bacterial strains were isolated from 8 different bat species across 11 locations in BC and Alberta. The identities of these anti-Pd bacteria are being determined.

Department

Biological Sciences

Faculty Advisor

Naowarat Cheeptham, Cori Lausen, and Jianping Xu

This document is currently not available here.

Share

COinS
 

Anti-Pseudogymnoascus destructans (Pd) Activities from Canadian Western Bat Wings

White-nose syndrome, an infectious fungal illness in bats caused by the psychrophilic Pseudogymnoascus destructans (Pd), has decimated North American hibernating bat populations at an unprecedented rate. The fungus establishes on the surface of bats during their hibernation cycle, resulting in their premature arousal from torpour and depletion of vital fat stores required for hibernation. As some species of bacteria have an antagonistic relationship with fungi, the elimination of Pd through the native microbiota of bats may serve as a non-invasive prevention method that limits the disruption of hibernacula ecosystems. In this study, bacteria found on batwings were isolated and screened for anti-Pd activity. These bacteria could be used to develop a probiotic powder to be placed in hibernacula and on bat colonies in hope to help prevent bats from WNS. Batwing swabs were obtained from captured bats between the summer of 2016 and the summer of 2018. The swabs were plated on blood agar, nutrient agar (NA), Reasoner’s 2A (R2A) agar, potato dextrose agar (PDA), and Wallerstein Laboratory (WL) agar. Culturable bacteria were re-streaked and incubated at 25-30°C for 24-48 hours to obtain pure cultures. Using the agar diffusion method, we screened the anti-Pd activity for our isolates on Sabouraud Dextrose agar (SDA) at 14°C for 28 days. Among the 585 bacterial isolates tested, five showed full inhibition and 34 showed partial inhibition. The anti-Pd bacterial strains were isolated from 8 different bat species across 11 locations in BC and Alberta. The identities of these anti-Pd bacteria are being determined.