Presentation Title

Determination of Epicatechin in Green Teas by UV Spectrophotometry

Format of Presentation

Poster to be presented the Friday of the conference

Presenter Information

Noor ShubearFollow

Abstract

Epicatechin is a polyphenolic catechin found in green tea leaves that has been shown to have antioxidant and anti-inflammatory properties. The current study will employ UV-visible spectrometry to quantify the levels of epicatechin in different green tea brands including an iced tea Japanese green tea, Stash green tea, Lipton mint green tea, Tetley green tea, and Arizona green tea with honey and ginseng. The study will employ random sampling to select the samples from the supermarket and one sample will used as a representative for each brand. All samples will be purchased from the same location. Calibration with external standards will be employed and used to generate a calibration curve. Five standard solutions with increasing concentrations of epicatechin will be prepared and sonicated to remove any suspended particulate that might interfere with the analysis, and these standard solutions will be analyzed in triplicate. The developed calibration curve will be used to determine the concentrations of epicatechin in the previously-mentioned tea brands. The method will be validated by calculating figures of merit for the analysis. Specifically, the limit of detection and limit of quantification will be calculated to evaluate the sensitivity of the method, and percent recovery to assess the accuracy of the method, and percent relative standard deviation to determine the precision of the analysis.

Department

Chemistry

Faculty Advisor

Kingsley Donkor

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Determination of Epicatechin in Green Teas by UV Spectrophotometry

Epicatechin is a polyphenolic catechin found in green tea leaves that has been shown to have antioxidant and anti-inflammatory properties. The current study will employ UV-visible spectrometry to quantify the levels of epicatechin in different green tea brands including an iced tea Japanese green tea, Stash green tea, Lipton mint green tea, Tetley green tea, and Arizona green tea with honey and ginseng. The study will employ random sampling to select the samples from the supermarket and one sample will used as a representative for each brand. All samples will be purchased from the same location. Calibration with external standards will be employed and used to generate a calibration curve. Five standard solutions with increasing concentrations of epicatechin will be prepared and sonicated to remove any suspended particulate that might interfere with the analysis, and these standard solutions will be analyzed in triplicate. The developed calibration curve will be used to determine the concentrations of epicatechin in the previously-mentioned tea brands. The method will be validated by calculating figures of merit for the analysis. Specifically, the limit of detection and limit of quantification will be calculated to evaluate the sensitivity of the method, and percent recovery to assess the accuracy of the method, and percent relative standard deviation to determine the precision of the analysis.