Presentation Title

Determination of Octanol-water Partition Coefficient for Indolicidin45 using Vesicular Electrokinetic Chromatography (VEKC)

Format of Presentation

Poster to be presented the Friday of the conference

Presenter Information

Puneet PariharFollow

Abstract

Antibiotic resistant bacteria (superbugs) are becoming an increasingly significant problem. There are new bacteria emerging that are completely resistant to the pharmaceutical industry's conventional antibiotics such as penicillin, cephalexin, etc. These superbugs are extremely dangerous as there is essentially no way to kill them, and many sicknesses have fatal results. In recent years, antimicrobial peptides (AMPs) have begun to garner interest. Bacteria have a very difficult time developing resistance to AMPs because they are very fast-acting. AMPs show a lot of potential to becoming a solution for the antimicrobial resistant pandemic that the future could hold. The AMP, indol45, an analog of indolicidin which has had the 4th and 5th tryptophan residues replaced with alanine, has shown increased antimicrobial activity along with reduced hemolytic activity when compared to indolicidin, making it a more viable candidate as an antimicrobial agent. The antibacterial properties of this peptide are still unknown. It is important to discover whether the bacteria are killed via lyses of its membrane or if the AMP enters the cell and kills it by a different mechanism. The aim of this research is to discover the answer through VEKC. VEKC is a variation of Micellar Electrokinetic Chromatography (MEKC), but is more adapted for larger molecules such as indolicidin45, and is therefore the ideal method. By determining the octanol-water partition coefficient, the ability of the drugs to pass through the membrane of bacteria can be determined. Determining the partition coefficient will help us establish the mode of action of indol45 and its derivative indolicidin’s antibacterial properties, clarifying its potential ability as a therapeutic antimicrobial agent.

Department

Other

Chemical Biology

Faculty Advisor

Heidi Huttunen-Hennelly

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Determination of Octanol-water Partition Coefficient for Indolicidin45 using Vesicular Electrokinetic Chromatography (VEKC)

Antibiotic resistant bacteria (superbugs) are becoming an increasingly significant problem. There are new bacteria emerging that are completely resistant to the pharmaceutical industry's conventional antibiotics such as penicillin, cephalexin, etc. These superbugs are extremely dangerous as there is essentially no way to kill them, and many sicknesses have fatal results. In recent years, antimicrobial peptides (AMPs) have begun to garner interest. Bacteria have a very difficult time developing resistance to AMPs because they are very fast-acting. AMPs show a lot of potential to becoming a solution for the antimicrobial resistant pandemic that the future could hold. The AMP, indol45, an analog of indolicidin which has had the 4th and 5th tryptophan residues replaced with alanine, has shown increased antimicrobial activity along with reduced hemolytic activity when compared to indolicidin, making it a more viable candidate as an antimicrobial agent. The antibacterial properties of this peptide are still unknown. It is important to discover whether the bacteria are killed via lyses of its membrane or if the AMP enters the cell and kills it by a different mechanism. The aim of this research is to discover the answer through VEKC. VEKC is a variation of Micellar Electrokinetic Chromatography (MEKC), but is more adapted for larger molecules such as indolicidin45, and is therefore the ideal method. By determining the octanol-water partition coefficient, the ability of the drugs to pass through the membrane of bacteria can be determined. Determining the partition coefficient will help us establish the mode of action of indol45 and its derivative indolicidin’s antibacterial properties, clarifying its potential ability as a therapeutic antimicrobial agent.