Investigating the Chemical Composition of Three Propolis Samples Using 1H Nuclear Magnetic Resonance (NMR) Spectroscopy

Wadiah Mubarak, Thompson Rivers University

Abstract

The resin found in propolis is known to have antimicrobial properties and for that reason it has been used since ancient times for many healing purposes. Flavonoids and aromatic acids are responsible for these antibacterial properties. Propolis is made up of natural resinous substances collected by honeybees from selective vegetal sources, which they then mix with their saliva. Honeybees synthesize the propolis and utilize it for various applications that are necessary for hive survival. Some of these uses include the antibacterial metabolites that provide defense against invaders, sealant for gaps in the hive, sterilization of the queen bee site so that the queen remains healthy, and mummifying insects that can not be carried out the hive. In this project, three propolis samples, isolated and obtained from different sources, are investigated for their differences and/or similarities in chemical composition and purity using 1H NMR spectroscopy. The antimicrobial assays for the propolis samples will not be conducted during this project because the propolis antibacterial activity has been previously identified against varieties of pathogenic microorganisms. However, I wanted experience with such assays so as an addition to my project on propolis, I have completed Kirby-Bauer assays for inodolicidin, an antimicoribal peptide isolated from bovine neutrophils, against selected pathogens. Indolicidin, a polycationic antimicrobial peptide has effective antimicrobial activities against a broad spectrum of microorganisms and multidrug-resistance organisms.

 

Investigating the Chemical Composition of Three Propolis Samples Using 1H Nuclear Magnetic Resonance (NMR) Spectroscopy

The resin found in propolis is known to have antimicrobial properties and for that reason it has been used since ancient times for many healing purposes. Flavonoids and aromatic acids are responsible for these antibacterial properties. Propolis is made up of natural resinous substances collected by honeybees from selective vegetal sources, which they then mix with their saliva. Honeybees synthesize the propolis and utilize it for various applications that are necessary for hive survival. Some of these uses include the antibacterial metabolites that provide defense against invaders, sealant for gaps in the hive, sterilization of the queen bee site so that the queen remains healthy, and mummifying insects that can not be carried out the hive. In this project, three propolis samples, isolated and obtained from different sources, are investigated for their differences and/or similarities in chemical composition and purity using 1H NMR spectroscopy. The antimicrobial assays for the propolis samples will not be conducted during this project because the propolis antibacterial activity has been previously identified against varieties of pathogenic microorganisms. However, I wanted experience with such assays so as an addition to my project on propolis, I have completed Kirby-Bauer assays for inodolicidin, an antimicoribal peptide isolated from bovine neutrophils, against selected pathogens. Indolicidin, a polycationic antimicrobial peptide has effective antimicrobial activities against a broad spectrum of microorganisms and multidrug-resistance organisms.