Presentation Title

Determination of Octanol-Water Partition Coefficient for Indolicidin45 Using Vesicular Electrokinetic Chromatography (VEKC).

Format of Presentation

Poster to be presented the Friday of the conference

Abstract

In response to the continued decline in effectiveness of conventional antimicrobial drugs, and the development of superbugs, researchers have begun to search for novel ways to address these problems. In recent years, antimicrobial peptides (AMPs) have begun to garner interest. Since AMPs have been shown to kill bacterial cells quickly, it is believed that it is very difficult for bacteria to develop resistance. This has caused many to believe that AMPs have the potential to become a solution for antimicrobial resistance. The AMP, indol45, an analog of indolicidin which has had the 4th and 5th tryptophan residues replaced with alanine, has shown increased antimicrobial activity along with reduced hemolytic activity when compared to indolicidin, making it a more viable candidate as an antimicrobial agent. However, it is unclear whether its antibacterial properties occur by simply lysing the membrane or by undergoing an intracellular mechanism. Therefore, the aim of this research is to answer this question using Vesicular Electrokinetic Chromatography (VEKC). VEKC has been used extensively as an indirect method for determining the octanol-water partition coefficient, which is the standard for determining a drugs ability to enter and pass through a membrane to reach its target site. Determining the partition coefficient will help us establish the mode of action of indol45’s antibacterial properties, elucidating its potential ability as a therapeutic antimicrobial agent.

Department

Chemistry

Faculty Advisor

Heidi Huttunen-Hennelly

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Determination of Octanol-Water Partition Coefficient for Indolicidin45 Using Vesicular Electrokinetic Chromatography (VEKC).

In response to the continued decline in effectiveness of conventional antimicrobial drugs, and the development of superbugs, researchers have begun to search for novel ways to address these problems. In recent years, antimicrobial peptides (AMPs) have begun to garner interest. Since AMPs have been shown to kill bacterial cells quickly, it is believed that it is very difficult for bacteria to develop resistance. This has caused many to believe that AMPs have the potential to become a solution for antimicrobial resistance. The AMP, indol45, an analog of indolicidin which has had the 4th and 5th tryptophan residues replaced with alanine, has shown increased antimicrobial activity along with reduced hemolytic activity when compared to indolicidin, making it a more viable candidate as an antimicrobial agent. However, it is unclear whether its antibacterial properties occur by simply lysing the membrane or by undergoing an intracellular mechanism. Therefore, the aim of this research is to answer this question using Vesicular Electrokinetic Chromatography (VEKC). VEKC has been used extensively as an indirect method for determining the octanol-water partition coefficient, which is the standard for determining a drugs ability to enter and pass through a membrane to reach its target site. Determining the partition coefficient will help us establish the mode of action of indol45’s antibacterial properties, elucidating its potential ability as a therapeutic antimicrobial agent.