Presentation Title

Dominant Effects of Septate Junction Protein Lachesin and Non-septate Junction Protein Akap200 in Hypomorphic Gliotactin genotypes with Defects in Frizzled-independent Planar Cell Polarity

Format of Presentation

Poster to be presented Friday March 31, 2017

Abstract

Epithelial sheets can be polarized in the apical-basolateral axis or within plane of the tissue. Polarization within the plane of an epithelium is referred to as planar cell polarity (PCP) and establishment of PCP is required for proper development. Drosophila melanogaster wing epithelia are a useful model system in the study of PCP because, during pupal development, Drosophila wing cells emit a single hair and the orientation of this hair can be used as an indicator for the individual cell’s PCP during development. Normal wing hair alignment is proximal to distal. Frizzled-signalling controls the global alignment of wing hairs and Frizzled-independent signalling controls local alignment. The first identified component of the Frizzled-independent system was Gliotactin. Several septate junction proteins, including Coracle, Discs-Large and Lachesin and one non-septate junction protein, Akap200, have also been shown to affect PCP. Recent research indicates that Akap200 localization is not affected in flies mutant in Gliotactin and Akap yet these flies exhibit enhanced winghair misalignment in comparison to Gliotactin mutations alone. This study investigates the effects of Akap and Lachesin mutations in a hypomorphic Gliotactin background on the proteins Akap, Lachesin and Gliotactin.

Department

Biological Sciences

Faculty Advisor

Dennis Venema

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Dominant Effects of Septate Junction Protein Lachesin and Non-septate Junction Protein Akap200 in Hypomorphic Gliotactin genotypes with Defects in Frizzled-independent Planar Cell Polarity

Epithelial sheets can be polarized in the apical-basolateral axis or within plane of the tissue. Polarization within the plane of an epithelium is referred to as planar cell polarity (PCP) and establishment of PCP is required for proper development. Drosophila melanogaster wing epithelia are a useful model system in the study of PCP because, during pupal development, Drosophila wing cells emit a single hair and the orientation of this hair can be used as an indicator for the individual cell’s PCP during development. Normal wing hair alignment is proximal to distal. Frizzled-signalling controls the global alignment of wing hairs and Frizzled-independent signalling controls local alignment. The first identified component of the Frizzled-independent system was Gliotactin. Several septate junction proteins, including Coracle, Discs-Large and Lachesin and one non-septate junction protein, Akap200, have also been shown to affect PCP. Recent research indicates that Akap200 localization is not affected in flies mutant in Gliotactin and Akap yet these flies exhibit enhanced winghair misalignment in comparison to Gliotactin mutations alone. This study investigates the effects of Akap and Lachesin mutations in a hypomorphic Gliotactin background on the proteins Akap, Lachesin and Gliotactin.