Presentation Title

"'Microbe's Digest': Gordonia sp. NB4-1Y and the Degradation of the Environmental Contaminant 6:2 fluorotelomer sulfonate (FTSA)"

Format of Presentation

Poster to be presented Friday March 31, 2017

Abstract

The purpose of our study was to determine the metabolites produced by the soil bacterium, Gordonia sp. NB4-1Y, during the degradation of the environmental contaminant and potential health hazard, 6:2 fluorotelomer sulfonate (FTSA). The Gordonia spp. possess the unique ability of metabolizing a wide variety of chemicals, specifically environmental contaminants. FTSA is one of many per- and poly-fluorinated chemicals (PFASs) known to persist in our surrounding environment and is suspected to have adverse health effects in animals and humans. Being one of the only labs to monitor the degradation of PFASs using microorganisms, our aim was to not only identify & quantify all metabolites produced, but to propose a potential metabolic pathway and identify the enzymes responsible for catalyzing breakdown. Our study would contribute to previous research by conducting the first analysis of FTSA degradation using Gordonia sp. NB4-1Y, identifying desulfonation and other enzymes involved in degradation, as well as taking the first step forward to finding a potential solution for the removal of the non-biodegradable compound FTSA from our environment. Experimental analysis of 336 samples derived from sulfur-limited pure cultures was determined using liquid chromatography coupled to mass spectrometry (LC-MS) in collaboration with McGill University, Montreal QC. Our research has identified & quantified 11 different metabolites, allowing us to propose a metabolic pathway. Furthermore, we are in the process of identifying the enzymes responsible for metabolism to occur. Future research will involve isolating these enzymes, verify their functionality, and cloning them for potential bioremediation.

Department

Biological Sciences

Faculty Advisor

Jonathan Van Hamme

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"'Microbe's Digest': Gordonia sp. NB4-1Y and the Degradation of the Environmental Contaminant 6:2 fluorotelomer sulfonate (FTSA)"

The purpose of our study was to determine the metabolites produced by the soil bacterium, Gordonia sp. NB4-1Y, during the degradation of the environmental contaminant and potential health hazard, 6:2 fluorotelomer sulfonate (FTSA). The Gordonia spp. possess the unique ability of metabolizing a wide variety of chemicals, specifically environmental contaminants. FTSA is one of many per- and poly-fluorinated chemicals (PFASs) known to persist in our surrounding environment and is suspected to have adverse health effects in animals and humans. Being one of the only labs to monitor the degradation of PFASs using microorganisms, our aim was to not only identify & quantify all metabolites produced, but to propose a potential metabolic pathway and identify the enzymes responsible for catalyzing breakdown. Our study would contribute to previous research by conducting the first analysis of FTSA degradation using Gordonia sp. NB4-1Y, identifying desulfonation and other enzymes involved in degradation, as well as taking the first step forward to finding a potential solution for the removal of the non-biodegradable compound FTSA from our environment. Experimental analysis of 336 samples derived from sulfur-limited pure cultures was determined using liquid chromatography coupled to mass spectrometry (LC-MS) in collaboration with McGill University, Montreal QC. Our research has identified & quantified 11 different metabolites, allowing us to propose a metabolic pathway. Furthermore, we are in the process of identifying the enzymes responsible for metabolism to occur. Future research will involve isolating these enzymes, verify their functionality, and cloning them for potential bioremediation.