Presentation Title

Annotation of Protein-Coding Genes in Drosophila ficusphila Genomic Contig 44 of the 3L Chromosome

Format of Presentation

Poster to be presented Friday March 31, 2017

Abstract

Genomics is a rapidly growing science. Through large-scale studies and analysis of large volumes of genomic data, the genome of Drosophila melanogaster has been well studied and completely annotated. Through a combination of various bioinformatics and comparative genomics tools, a closely related species such as D. ficusphila can be annotated. These tools, among others, are available through the Genomics Education Partnership and NCBI, which include a Genome browser, BLAST, Gene Record Finder, and Gene Model Checker. Genomic comparisons of the unannotated D. ficusphila genome to the well studied D. melanogaster genome allows for identification and annotation of genes within genomic sequences. In this study, protein-coding genes were manually annotated via genome browsing and comparison to the D. melanogaster genome. The coordinates for all protein coding exons and splice sites of putative gene orthologs in D. ficusphila were annotated and checked via the Gene Model Checker. Overall, 4 putative gene orthologs with a combined total of 21 isoforms in the 39000 base pair 'contig 44' in D. ficusphila were identified. Gene models for each gene and isoform were constructed within this contig and putative locations of transcriptional start sites were investigated.

Department

Biological Sciences

Faculty Advisor

James E. J. Bedard

This document is currently not available here.

Share

COinS
 

Annotation of Protein-Coding Genes in Drosophila ficusphila Genomic Contig 44 of the 3L Chromosome

Genomics is a rapidly growing science. Through large-scale studies and analysis of large volumes of genomic data, the genome of Drosophila melanogaster has been well studied and completely annotated. Through a combination of various bioinformatics and comparative genomics tools, a closely related species such as D. ficusphila can be annotated. These tools, among others, are available through the Genomics Education Partnership and NCBI, which include a Genome browser, BLAST, Gene Record Finder, and Gene Model Checker. Genomic comparisons of the unannotated D. ficusphila genome to the well studied D. melanogaster genome allows for identification and annotation of genes within genomic sequences. In this study, protein-coding genes were manually annotated via genome browsing and comparison to the D. melanogaster genome. The coordinates for all protein coding exons and splice sites of putative gene orthologs in D. ficusphila were annotated and checked via the Gene Model Checker. Overall, 4 putative gene orthologs with a combined total of 21 isoforms in the 39000 base pair 'contig 44' in D. ficusphila were identified. Gene models for each gene and isoform were constructed within this contig and putative locations of transcriptional start sites were investigated.