Presentation Title

The Molecular Binding of Resveratrol to Human Serum Albumin using Affinity Capillary Electrophoresis

Format of Presentation

Poster to be presented Friday March 31, 2017

Abstract

Resveratrol is a type of natural phenol which is found in the skin of grapes, blueberries and raspberries which is gaining popularity in the health field as it is believed to have anti-cancer, anti-inflammatory, cardio-protective and chemo-preventative effects. Due to the low aqueous solubility of resveratrol, a delivery protein must be used to transport resveratrol from the plasma to the cell-active site. It is hypothesized that human serum albumin (HSA) is the major protein which allows for the transfer of resveratrol into the blood plasma; therefore, it would be essential to perform binding studies between resveratrol and HSA. In this work, an affinity capillary electrophoresis method was developed to determine the binding constant which correlates to the strength of the binding between resveratrol and HSA. In addition, a thermodynamic study was also performed to determine the type of non-covalent binding between the two molecules.

Department

Chemistry

Faculty Advisor

Kingsley Donkor

This document is currently not available here.

Share

COinS
 

The Molecular Binding of Resveratrol to Human Serum Albumin using Affinity Capillary Electrophoresis

Resveratrol is a type of natural phenol which is found in the skin of grapes, blueberries and raspberries which is gaining popularity in the health field as it is believed to have anti-cancer, anti-inflammatory, cardio-protective and chemo-preventative effects. Due to the low aqueous solubility of resveratrol, a delivery protein must be used to transport resveratrol from the plasma to the cell-active site. It is hypothesized that human serum albumin (HSA) is the major protein which allows for the transfer of resveratrol into the blood plasma; therefore, it would be essential to perform binding studies between resveratrol and HSA. In this work, an affinity capillary electrophoresis method was developed to determine the binding constant which correlates to the strength of the binding between resveratrol and HSA. In addition, a thermodynamic study was also performed to determine the type of non-covalent binding between the two molecules.