Presentation Title

A Forward Mutagenesis Screen on a Gliotactin Hypomorphic Background in Drosophila Melanogaster: Isolation and Characterization of ∇11

Format of Presentation

Poster to be presented Friday March 31, 2017

Abstract

Planar cell polarity is a phenomenon observed in Drosophila where epithelial cells are polarized within the plane of the cell layer during development. Wing hair on Drosophila exemplify both global and local polarity in their orientation and parallel arrangement. Global polarity is controlled by the frizzled signaling pathway, and local polarity is controlled by the frizzled-independent signaling pathway. Mutations in the septate junction (SJ) protein gliotactin (gli) show disruption in local polarity, known as the wing hair misalignment phenotype. Mutations in several other SJ and non-SJ proteins also produce the wing hair misalignment phenotype. A forward mutagenesis P-element screen was performed on a gli hypomorphic background, and ∇11 was determined to be an enhancer of the wing hair misalignment phenotype. Inverse PCR will be used to determine the location of ∇11, and a series of test crosses will be performed to determine its effect on the lethality and wing hair misalignment phenotypes.

Department

Biological Sciences

Faculty Advisor

Dennis Venema

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A Forward Mutagenesis Screen on a Gliotactin Hypomorphic Background in Drosophila Melanogaster: Isolation and Characterization of ∇11

Planar cell polarity is a phenomenon observed in Drosophila where epithelial cells are polarized within the plane of the cell layer during development. Wing hair on Drosophila exemplify both global and local polarity in their orientation and parallel arrangement. Global polarity is controlled by the frizzled signaling pathway, and local polarity is controlled by the frizzled-independent signaling pathway. Mutations in the septate junction (SJ) protein gliotactin (gli) show disruption in local polarity, known as the wing hair misalignment phenotype. Mutations in several other SJ and non-SJ proteins also produce the wing hair misalignment phenotype. A forward mutagenesis P-element screen was performed on a gli hypomorphic background, and ∇11 was determined to be an enhancer of the wing hair misalignment phenotype. Inverse PCR will be used to determine the location of ∇11, and a series of test crosses will be performed to determine its effect on the lethality and wing hair misalignment phenotypes.