Presentation Title

Chemical Control of Crown Gall Disease in Grapevines at Harper’s Trail Winery, Kamloops, B.C.

Presenter Information

TingYun Shiue

Location

House of Learning Library, 3rd floor

Start Date

18-3-2016 12:00 PM

End Date

18-3-2016 6:00 PM

Abstract

Crown gall, a significant grapevine disease found worldwide, is caused by tumor-inducing bacteria, Agrobacterium vitis. A. vitis is only found on grape, and commonly causes galls on the lower trunks, or at graft unions, of grapevines. During infection, once the tumorous gene is transferred to the plant, the presence of the bacteria is not necessary. Therefore, removal of galls does not eliminate the disease. Since the infection of A. vitis is systemic, it is difficult to find a method to eliminate the disease without harming the plant. Harper’s Trail Winery, in Kamloops, B.C., has found that their grapevines were infected by A. vitis which causes death of grapevines and economic losses. The present study is to find a potential treatment in order to kill the bacteria and prevent future infection. Samples of A. vitis were isolated from the galls collected from Harper’s Trail Winery in the summer of 2015. The bacteria were identified preliminary by microscopic and macroscopic morphologies. A growth curve was determined for screening. Three different concentrations of colloidal silver, which has been used on plants to produce more and healthier crops, were used to examine if the A. vitis can be inhibited by treatment of colloidal silver. The screening has not been completed, but the predicted result is that the growth of A. vitis will be inhibited by the highest concentration of colloidal silver, although it remains unknown whether it can still work while treating the actual plants in the field.

Department

Biological Sciences

Faculty Advisor

Naowarat Cheeptham

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Mar 18th, 12:00 PM Mar 18th, 6:00 PM

Chemical Control of Crown Gall Disease in Grapevines at Harper’s Trail Winery, Kamloops, B.C.

House of Learning Library, 3rd floor

Crown gall, a significant grapevine disease found worldwide, is caused by tumor-inducing bacteria, Agrobacterium vitis. A. vitis is only found on grape, and commonly causes galls on the lower trunks, or at graft unions, of grapevines. During infection, once the tumorous gene is transferred to the plant, the presence of the bacteria is not necessary. Therefore, removal of galls does not eliminate the disease. Since the infection of A. vitis is systemic, it is difficult to find a method to eliminate the disease without harming the plant. Harper’s Trail Winery, in Kamloops, B.C., has found that their grapevines were infected by A. vitis which causes death of grapevines and economic losses. The present study is to find a potential treatment in order to kill the bacteria and prevent future infection. Samples of A. vitis were isolated from the galls collected from Harper’s Trail Winery in the summer of 2015. The bacteria were identified preliminary by microscopic and macroscopic morphologies. A growth curve was determined for screening. Three different concentrations of colloidal silver, which has been used on plants to produce more and healthier crops, were used to examine if the A. vitis can be inhibited by treatment of colloidal silver. The screening has not been completed, but the predicted result is that the growth of A. vitis will be inhibited by the highest concentration of colloidal silver, although it remains unknown whether it can still work while treating the actual plants in the field.