Presentation Title

Preliminary Screening of New Brunswick Cave Bacteria for Antimicrobial Activity against Pathogenic Bacteria

Presenter Information

Sarah Gomes

Location

House of Learning Library, 3rd floor

Start Date

18-3-2016 12:00 PM

End Date

18-3-2016 6:00 PM

Abstract

Antibiotic-resistant bacteria are an increasing threat to public health, and have led to an urgent need for new antibiotics. Caves can be great sources for new drug discovery as little is known about bacteria that live in these environments. Our goal was to find bacteria from caves that may be able to produce bioactive compounds against multidrug-resistant bacteria, as a foundation to find new antibiotics. This study was conducted using bacteria from seven New Brunswick caves with varying environmental conditions. Soil samples from each cave were diluted and inoculated onto three media types: R2A, Hickey-Tresner and Actinomycete isolation. A total of 795 cave bacteria were isolated and tested against selected pathogenic bacteria as a preliminary screening for antagonistic interactions. Screening of cave bacteria against the target bacteria showed that 31 reacted against Escherichia coli, 187 reacted against a multidrug resistant Escherichia coli-318, and 403 reacted against Pseudomonas aeruginosa. The interactions observed were mainly zones of inhibition; however, some bacteria induced the growth of the pathogenic bacteria. Certain caves showed a higher bacterial diversity than others and bacteria grown on R2A media exhibited the highest diversity to interact with the pathogenic bacteria. Thus far, no bacteria have been identified. This research lays the groundwork for discovering bacteria that can potentially be beneficial in advancing current antibiotics.

Department

Biological Sciences

Faculty Advisor

Naowarat Cheeptham

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Mar 18th, 12:00 PM Mar 18th, 6:00 PM

Preliminary Screening of New Brunswick Cave Bacteria for Antimicrobial Activity against Pathogenic Bacteria

House of Learning Library, 3rd floor

Antibiotic-resistant bacteria are an increasing threat to public health, and have led to an urgent need for new antibiotics. Caves can be great sources for new drug discovery as little is known about bacteria that live in these environments. Our goal was to find bacteria from caves that may be able to produce bioactive compounds against multidrug-resistant bacteria, as a foundation to find new antibiotics. This study was conducted using bacteria from seven New Brunswick caves with varying environmental conditions. Soil samples from each cave were diluted and inoculated onto three media types: R2A, Hickey-Tresner and Actinomycete isolation. A total of 795 cave bacteria were isolated and tested against selected pathogenic bacteria as a preliminary screening for antagonistic interactions. Screening of cave bacteria against the target bacteria showed that 31 reacted against Escherichia coli, 187 reacted against a multidrug resistant Escherichia coli-318, and 403 reacted against Pseudomonas aeruginosa. The interactions observed were mainly zones of inhibition; however, some bacteria induced the growth of the pathogenic bacteria. Certain caves showed a higher bacterial diversity than others and bacteria grown on R2A media exhibited the highest diversity to interact with the pathogenic bacteria. Thus far, no bacteria have been identified. This research lays the groundwork for discovering bacteria that can potentially be beneficial in advancing current antibiotics.